GB 5009.285-2022 is applicable to the determination method of vitamin B12 in food. Compared with GB 5413.14-2010,it revised the original microbiological method to the third method as the microbiological method, added the first method of liquid chromatography and the second method of liquid chromatography as mass spectrometry.

Contents

1. Scope

2. Principle

3. Reagents and materials

4. Instruments and equipments

5. Analysis steps

6. Formulation of analysis results

7. Precision

8. Others

Appendix A Method of immunoaffinity column reference validation 

Appendix B Liquid chromatogram

Appendix C Liquid chromatography-mass spectrometry

Appendix D Method of medium preparation